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Description

CRISPR-Cas systems restrict horizontal gene transfer in clinical Staphylococcus aureus strains

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E.HoutsG

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GATA1 overexpression (HMD-GATA1-IRES-GFP) and control plasmids have been described previously.26 Lentiviruses were packaged in HEK293T cells according to the manufacturers protocol (Invitrogen, MA, USA)

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The cells were transfected using 500 nM of the negative control or specific siRNAs

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Learn more about Polysorbate 20 Dimethyl Isosorbide (often shortened to DMI) is a sugar-derived solvent made from sorbitol

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BMC Pediatr 21(1):178

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