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The metabolite concentrations in tissues or intestinal contents were calculated using the following equation: concentration (mol g 1 ) = concentration of extracted sample (M) volume of extraction solution (l) / tissue weight (mg)

doi:10.1038/nprot.2006.59

doi:10.1002/ange.202004094

For antigen retrieval, the tissue sections were treated with antigen retrieval solution pH 9 (Dako, Via Real, CA, USA) and heated

Using the wrong syringe scale for your calculations results in dosing errors of up to 2.5 times the intended amount

In clinical studies, pharmacological supplementation of BH4 improves endothelium-dependent relaxations and augments NO-mediated effects on forearm blood flow in smokers and those with diabetes and elevated cholesterol [8588]