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To do so, cell lysates from either HepG2 cells or mouse thymocytes were treated with various doses of LC and positive control agents

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Structure determination was carried out using a ThermoFisher Titan Krios TEM operating at 300 kV equipped with a Gatan BioQuantum energy filter (with a 15 eV slit width for OCTN2 inward, OCTN2 ipratropium and a 25 eV width for OCTN2 carnitine), and a Gatan K3 camera