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Collectively, to systematically investigate tissue- and cell-type-specific expression profiles of E3 Ub ligases, under normal or diseased/stressed states, will be an attracting topic in the next decade either to comprehensively understand the biology of E3 ligases in different diseases/stresses or to extend PROTAC applications

Key areas requiring further investigation include cumulative effects of chronic exposure, drug interactions, and impact on various organ systems

doi: 10.1146/annurev-phyto-020620-114550 81 ZechmannB

Novel surface modification techniques have enhanced targeting capabilities, and recent studies have explored cell-penetrating peptides, aptamers, and receptor ligands to improve cellular uptake and tissue specificity