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The flow rate was maintained at 0.15 ml min 1 with the following linear gradient: 023 min from 95% buffer B to 5% buffer B, 2325 min hold at 5% buffer B, 2525.5 min transition to 95% buffer B at 0.20 ml min 1 , 25.532.5 min hold at 95% buffer B and 32.533 min at 95% buffer B with a 0.15 ml min 1 flow rate

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ROS can also cause an imbalance in mitochondrial metabolism, causing change in membrane permeability and halting the ATP production, which in turn cause cell cycle alterations, ultimately leading to the cancer appearance (Figure 2) [21]