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The digested products were then diluted by adding 100 mM ammonium bicarbonate buffer to 1 ml

This has been shown to happen in soda and other products that also contain vitamin C

Luo LY, He HY, Li CH, et al

The lower ratio in primed cells could partially suppress TET/JMJ demethylation, helping maintain the higher DNA/histone methylation characteristic of primed pluripotency, whereas the higher ratio in naive cells permits more active demethylation and a hypomethylated state