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More importantly, in this study, it is strongly confirmed, for the first time, that AST could protect the mitochondrial structure, inhibit ferroptosis, and activate autophagy through the SLC7A11/GPX4 signaling pathway against DED in vivo and in vitro The effect of astaxanthin in hyperosmolarity-induced corneal epithelium cells was summarized in Figure 11

(A) The optic disk is a normal shape, with a normally sized neuroretinal rim (superior, inferior, nasal and temporal)

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Similarly, TEM showed that the number of autophagosomes increased in HCECs in the AST + DE group compared with the DMSO + DE group (Figure 8G)