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Similar approaches in other species have confirmed that SPDS is a robust engineering target for boosting spermidine-linked stress resilience 87,88

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Similarly, the expression of a constitutively activated form of GSK3 (p.S9A mutant) in HepG2 cells induced a significant decrease of H 2 S production (Figure 2G) without affecting cell proliferation and/or viability (Supplementary Figure 1E)