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Under physiological conditions, a healthy gut microbiota and an intact intestinal barrier are critical for establishing immune tolerance ( The tumor immune microenvironment (TIME), a dynamic ecosystem of cancer cells, stromal cells, ECM, and diverse immune cells, profoundly shapes tumor progression and therapeutic responses ( A pivotal mechanism linking gut dysbiosis to pro-tumorigenic inflammation is the translocation of microbial components, most notably lipopolysaccharide (LPS), across a compromised intestinal barrier ( Beyond TLR-dependent microbial sensing, the NF-B pathway serves as a critical signaling hub that integrates diverse pro-tumorigenic stimuli within the TIME

Similarly, astrocytes are recognized to change their shape, behavior, and gene activity in response to injury, disease, or infection in CNS

More than 70% of patients with variants of ABCB11 and ABCB4 had fibrosis [22]

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It also lowers the risk of bacterial growth compared to plain sterile water