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Cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum, 100 g/mL penicillin/streptomycin/gentamicin (Invitrogen, Gibco) and in the condition of 95% air and 5% CO 2 humidified atmosphere at 37 C

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Meanwhile, HFD reduced FBW, BWG, and PER while raised FCR, IPF, liver fat %, and body ash %, but reduced body protein% and fat %

Table 3 reports the translation bile acid biomarkers of APAP-induced hepatotoxicity including the number of human subjects, biofluid and method validation method

Acoustic waves physically break up these barriers