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Dear TN, Kefford RF: The WDNM1 gene product is a novel member of the 'four-disulphide core' family of proteins

Tissue sections or fixed endothelial cells were processed for immunolabeling by permeabilization (0.4% Triton X-100 in PBS), blocking (4% horse serum, 2% BSA, 0.2% Triton X-100 in PBS) and incubation with first antibody (1% HS, 0.05% Triton X-100 in PBS)

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Kutyba J, Jedrzejczak WW, Gos E, Bienkowska K, Raj-Koziak D, Skarzynski PH

Also the areas of demyelinization were noted, especially in the cerebellum

All dosing is speculative because rat studies used intraperitoneal administration that doesnt directly translate to human routes