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doi: 10.1080/15563650.2017.1291945 [PubMed] 29423816 Yarema M, Chopra P, Sivilotti MLA, Johnson D, Nettel-Aguirre A, Bailey B, Victorino C, Gosselin S, Purssell R, Thompson M, Spyker D, Rumack B

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Then the gene expression was quantified dependent on the LightCycler480 PCR system (Roche) by using 2SYBR Green qPCR Master Mix (biotool, #B21203)