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Proteins were transferred to PVDF membranes (Thermofisher) using a Power BlotterSemi-dry Transfer System (Thermofisher) and incubated overnight with IFN- (E3V1X) Rabbit mAb (Cell Signaling, #98139S), Galectin-1/LGALS1 (D608T) Rabbit mAb (Cell Signaling, #12936S, Granzyme B (E5V2L) Rabbit mAb (Cell signaling, #44153) and SHMT2 (E7F4Q) Rabbit mAb (Cell signaling, #3344), vinculin Mouse mAb (Sigma-aldrich, #V9131), actin (clone C4) Mouse mAb (MP biomedicals, #691001) antibodies diluted in 5% skimmed milk in Tris-Buffered NaCl Solution with Tween 20 (TBST)

The resulting metabolic stress triggers phosphorylation cascades through several pathways

Eknoyan G

In fact, it was recently found that NNMT participates in regulating stem cell pluripotency in hESCs

Koike K, Kawabe T, Tanaka T, Toh S, Uchiumi T, Wada M, Akiyama S, Ono M and Kuwano M

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