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Primers were identified and removed using cutadapt (v2.7) with a maximum error rate of 0.2, reads lacking both forward and reverse primers were discarded

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AlphaPlex is a further development that allows quantification of up to three analytes by using different acceptor beads that emit at distinct wavelengths

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Moreover, the pathological mechanisms of these endothelial injuries seem to be attributed to disorders of the NO/cGMP pathway, abnormal endothelium-derived vasoactive factors, oxidative stress, etc

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