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Having incubated the immunoprecipitated samples overnight at 4 C with rotation, the anti-M2 FLAG antibody-conjugated agarose beads were washed three times (each for 5 min) with ice-cold washing buffer (50 mM Tris, pH 7.4, 137 mM NaCl, 10% glycerol, 1% NP-40)

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The research team then calculated the wet/dry ratio of each specimen in order to compare them to the values of the control groups

SLC39A14, a member of the same solute carrier family, facilitates Mn uptake in the liver for excretion into bile

Exclusion criteria included: (1) studies involving populations other than adult patients undergoing hemodialysis

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