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To mimic an overnight fast in humans, the mice were fasted for 4 hours in the morning 79 , after which initial blood glucose levels were measured

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After 30 min of puromycin treatment, cells were lysed in RIPA Lysis and Extraction Buffer (Cat# 89901) that included Halt Protease Inhibitor Cocktail (Cat# 78430) and Halt Phosphatase Inhibitor Cocktail (Cat# 78420) from Thermo Fisher Scientific (Waltham, MA, USA)

Is one B12 shot a month enough