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While there are nine essential amino acids, L-leucine is widely regarded as the master regulator of protein synthesis [1]

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Differentially expressed metabolites ( Table S2 ) were identified by statistical analysis (VIP 1 & p.adj 10, n = 20) groups

Solid-phase peptide synthesis (SPPS), native chemical ligation (NCL) and desulfurization Peptides were synthesized by standard Fmoc-strategy on a ResPep SLi (Intavis) parallel synthesizer using Fmoc- and sidechain-protected amino acids in either l- or d-configuration obtained from Carbolution

Nutrient Dosing: In some applications, nutrients (such as nitrogen or phosphorus) are dosed to support microbial activity and prevent biofilm starvation

Ions were added using the gmx genion tool to neutralize the system