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Mancuso M, Orsucci D, et al

Exp Neurol 257:105113

The reaction of rChTg (5 M) toward NBD-Cl (50 M) was determined spectrophotometrically at 419 nm were the Cys-NBD adduct absorbs ( = 13000 M 1 cm 1 ) 29 in 50 mM sodium acetate buffer pH 5.0, 0.28 M urea (25 C)