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Following further centrifugation, supernatants were added to pre-prepared anti-GFP (19C8 and 19F7 antibodiesSloan Kettering Memorial Centre) coated magnetic beads (Dynabeads MyOne Streptavadin T1Life Technologies)

For example, some studies have found that the secondary mutations of D1228N/V and Y1230C in the MET kinase domain are the main mechanisms for acquired resistance to crizotinib in METex14 patients receiving c-MET inhibitor treatment [149, 150]

In this context, even modest alterations in angiogenesis, inflammatory signaling, or coagulation balance, as well as contamination exposure, may be clinically meaningful