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WN and KZ designed and formulated the review theme and revised and finalized the manuscript

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Cost-saving by using less imidazole During purification of His-tagged proteins with conventional Ni-NTA and Ni-IDA based affinity resins, imidazole is generally required to include in the binding and wash buffers to modulate non-specific binding

Detection was performed using an enhanced chemiluminescent reagent (Millipore)

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What is the difference between Methylcobalamin and Hydroxycobalamin B12