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The instrument was calibrated using 0, 5, 10, 50, 100 and 500 ppb of certified multi-element ICP-MS standard calibration solutions (ICP-MS-CAL2-1, ICP-MS-CAL-3 and ICP-MS-CAL-4, Accustandard) for a range of elements

This complex includes the nucleus tractus solitarius (NTS), the area postrema (AP), and the dorsal motor nucleus of the vagus nerve (DMV)

3.4 Cytotoxicity assay The cytotoxicity of SBA-15 MSN, FA-SNCD@SBA-15, FA-SNCD@SBA-15/ENZ and free ENZ in PC3 cell lines was evaluated using the MTT assay (to assess in vitro toxicity)