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(5) PRODH in the regulation of ferroptosis Potential links between proline metabolism and ferroptosis PRODH is a mitochondrial enzyme primarily responsible for the oxidation of proline to P5C as part of amino acid catabolism and redox metabolism 101

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Its rigid lipophilic phenanthrenequinone skeleton enables it to rapidly enter macrophages and specifically target and block the cytoplasmic ROCK2/NF-B signaling axis (Zhang et al., 2025)

Inflammatory modulation: animal models of experimental colitis (TNBS, DSS), toxic hepatitis and joint damage documented reduction of pro-inflammatory cytokines (TNF-, IL-6, IL-1) and improvement of histological lesion scores

Additionally, we used Prussian blue staining for the detection of ferric iron (Fe 3+ ) accumulation in brain tissue