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Studies of cultured human vascular endothelial cells (HUVECs) have demonstrated that competitive inhibition of G6PD in the HUVECs using 6-aminonicotinamide (6-AN) or G6PD knockdown downregulate eNOS activity and nitrate levels

Catalase is another important enzyme to reduce intracellular ROS levels

The solution was applied to the calcium fluoride window slice using a plastic dropper, dried in an oven to remove the effect of moisture, and fixed in a liquid cell for infrared spectroscopy

This harvested biomass of fungal cells was centrifuged at 16,000 g for 5 minutes 80 .The tubes and bottles were treated with 0.1% DEPC solution at 37 C overnight, autoclaved twice at 121 C for 20 min and then dried at 100 C before use