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Sigma Aldrich, Milwaukee, WI), a laparotomy was performed, and both renal pedicles were clamped for 22 min

We evaluated two biological processes (aging and apoptosis) and club host defense in club cells

Nature 2017;548:1126

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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation