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For intracellular staining (ICS) 5,000,000 cells per well were plated in a 96-well round bottom plate (Costar 3799) and stimulated using the same ELISpot conditions as described above and incubated at 37C with 5% CO 2 for a total of 5.5h

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Selenium from selenium yeast In this process, yeast is grown on selenium-rich culture media and then dried