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Metabolic engineering of ketocarotenoids biosynthesis in the unicelullar microalga Chlamydomonas reinhardtii

Apart from the redox status and ROS detection spin probes offer, via their EPR spectra, information on their mobility and different characteristics of their environment such as viscosity, pH, p O 2 , and temperature (Kocherginsky and Swartz, CHAs, such as TMT-H and DCP-H, become paramagnetic after oxidation, are EPR-silent and are reduced equimolarly by several ROS into EPR-active nitroxides

Editors JorgensenS.FathB

Agrobacteria containing pTRV1 and pTRV2 or pTRV2 derivative plasmids were grown overnight at 28 C

Furthermore, RSV (5 mg/kg) has been shown to extend platelet plug development in mice (207), implying there is a tendency to bleed when using it

In all, 5 g pMT3-GATA-1 (full length) was transfected into COS-7 cells (gift from Stu Orkin, Harvard Medical School Boston, USA) for 48 h using Fugene6 (Promega, #E2691)