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The median age of the patients was 54 years (IQR, 50-60 y) and the majority of them were male (509 [82.9%])

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For microglia, the DAPI and Cy3(IBA-1) channels were merged, and for astrocytes, the DAPI, Cy3(GFAP), and Cy2 (S100) channels were merged via the Colour and Merge Channels tools

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Financial support and sponsorship This work was supported by NIH grant R01 AG057381, Ladiges, PI