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Conclusion Cu(II)-binding affinities for three major blood copper proteins: human serum albumin, ceruloplasmin and alpha-2-macroglobulin, were studied through their competition with a set of low-molecular weight Cu(II)-binding reference ligands (DTPA, EDTA, NTA, His) by using an unified LC-ICP MS-based approach

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Ethics declarations Competing interests The authors declare no competing interests

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