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The eluates were recovered and neutralized to pH: 6.5 using 0.1 mL of buffering solution [tris(hydroxymethyl)aminomethane solution at pH: 8.5] and stored at 80C until antibody analysis

The dihexa half life determines clearance speed

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In both BacA and wild-type groups the survival rate of MAP in macrophages alone was significantly higher than macrophages incubated with PBMCs, regardless of PBMCs being primed ( Figure 8B ), suggesting that effector T cells contribute to the killing of MAP