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A plausible explanation is that rapamycin only partially inhibits phosphorylation of certain downstream mTORC1 substrates, with some of the 4E-BP1 phosphorylation sites being largely unaffected (Kang et al., 2013)

Following experimental treatments, cells are harvested, washed, and lysed according to the kit manufacturers protocol

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& Padhan, P

Firstly, autophagy can collaborate with apoptosis to trigger cell death, which often occurs in cytotoxic autophagy in chemotherapy

Yellowing can be induced by factors including nutritional stress, pathogenic infection, hormonal balance, water loss, temperature fluctuations, energy consumption, metal ion imbalance, and cell decay