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The tissues were homogenized in ice-cold buffer, centrifuged for 30 min at 10,000 g, and the supernatant was kept at -80C for neurotransmitter (ACh, AchE, GABA, 5-HT, and dopamine), oxidative stress markers (MDA), and antioxidant status (GSH, CAT, and T-SOD) determinations

These resources include: A CISP Checklist Implementing Revised List (available now - originally published January 2021) Athlete and ASP Guide to the 2025 List (available now) Languages and Formats The 2025 Prohibited List, the 2025 Summary of Modifications and Explanatory Notes, and the 2025 Monitoring Program are available for download on WADAs website in English, French, and Spanish