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These insights help guide therapy selection, monitor response and refine peptide protocols over time

During in vivo experiments, WT BL6 mice received three intravenous injections of either transfected or non-transfected cells, followed by B16-OVA melanoma cell implantation

In line with the covalent binding mechanism, in vitro and cell-based thermal-shift assays showed that C1-27 induced a negative shift in the melting temperature indicating protein destabilization on binding (Supplementary Figs 11 and 15)