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[119] It is important to remember, however, that charge alterations associated with various SOD1 mutations will differ depending on the pH of the subcellular compartment within which SOD1 resides, as well as the maturation/metalation state of SOD1 within that compartment.[ 77 , 119 ] These biophysical changes are consistent with the exposure of hydrophobic residues located in the SOD1 dimer interface, amongst other conformational changes, and may be indicative of consistent dimer instability

For glioblastoma (GBM), a blood-brain barrier-penetrating delivery system was developed using Angiopep-2/TAT peptide-modified extracellular vesicles (EVs) to transport Cas9/sgRNA complexes targeting glutathione synthetase (GSS)