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The chamber is designed to separate the luminal side from the basolateral side of the epithelium and, to assess permeability, molecules are placed in the luminal chamber, and the flux of these markers to the basolateral side is measured as an indicator of permeability of the epithelium [41]

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2.4 Elevated plus maze test Anxiety-related behavior was evaluated using a standard elevated plus maze (EPM) with two open and two closed arms intersecting at a central platform

Here, we provide a systematic discussion of these mechanism by classifying them in alterations (1) that involve steps preceding the binding of cisplatin to DNA (pre-target resistance), (2) that directly relate to DNAcisplatin adducts (on-target resistance), (3) concerning the lethal signaling pathway(s) elicited by cisplatin-mediated DNA damage (post-target resistance) and (4) affecting molecular circuitries that do not present obvious links with cisplatin-elicited signals (off-target resistance)

Assessing level of evidence is important for clinicians and this tool kit was used to provide a sense of the strength of recommendations based on a body of evidence

Gluten-Free, Casein-Free Diets for Autism Put to the Test Are Autism Diet Benefits Just a Placebo Effect