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Expression of RCC1 (a GEF for Ran) reversed the reduction in RanGTP/total Ran ratio induced by Sema4D treatment in MCF7 and LNCaP cells (Fig

With an average degree of damage: apply half of the above dose

In addition, for NSBSO treated B16 cells, the expression of GPX4 did not have obvious change, but the expression of GCL decreased obviously (Additional file 1: Figs

Sequences of forward primer were 5-GGA AGATCT TCCGCCAAAGATGTGGTGGTG-3 with Bgl II restriction site and reverse primer were 5-CG CTCGAG CGGTTGCAAATCCGCGTC TTCAAAG-3 with Xho I restriction site (bold sequences represent the restriction sites)

In the current study, our results suggest that GPX1 protects against peroxynitrite-induced SGNs damage by suppressing ROS accumulation in SGNs