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We generated three constructs, the full-length ElpQ lacking the native signal sequence (aa 19-343), along with N-terminal (aa 19-206) and C-terminal (aa 168-343) truncation constructs that were termed ElpQ FL, ElpQ N, and ElpQ C, respectively

However, realistic expectations are important

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J Biol Chem 279: 5534155347 JoSH,SonMK,KohHJ,LeeSM,SongIH,KimYO,LeeYS,JeongKS,KimWB,ParkJW,SongBJ,HuhTL (2001) Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+dependent isocitrate dehydrogenase

An adequate and hopefully incisive therapeutic action will therefore depend on a finely orchestrated balance between the efficacy of the single treatment for the specific pathogenetic mechanism and the control of potentially noxious interactions among the different therapies