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S-IP, J-JK, and K-IL performed the experiments

For that, labeled molecules with radioactive isotopes or with fluorophores applied at the luminal side are commonly used

Pahwa, Rajesh|Lyons, Kelly E|Hauser, Robert A Ropinirole therapy for Parkinsons disease Expert Review of Neurotherapeutics

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Buffer EB (Qiagen) was used to resuspend the purified DNA

Supernatants were applied to a HisTrap FF column and eluted with a linear gradient of imidazole, then further purified using a Superdex 200 column