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Target sequences, which were cloned from a rat cDNA library, were inserted into pENTR-1A vector (cytoGPx4-Ad, attL1-SalI-kozak-GPx4 [cyto]-FLAG-SECIS-NotI-attL2

Protein injury can be quantified via carbonyls (DNPH derivatization), nitrotyrosine as an RNS-linked marker and advanced oxidation protein products, with MS-based identification/validation wherever feasible to improve specificity and DNA damage should include 8-oxoG, abasic sites and strand breaks, interpreted cautiously because activation of repair pathways (BER/PARP) can secondarily reshape metabolic readouts (e.g., NAD/ATP depletion) (Weber et al

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Moreover, HIF expression has been observed in chondrocytes and osteoclasts, indicating its ubiquitous presence in synovial tissues (129)

Inhibition or activation effects are indicated by - or +, respectively