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In the above reaction, Glutathione Peroxidase is the rate-limiting step of the entire reaction system, so the decrease in NADPH is linearly related to the activity of Glutathione Peroxidase

10.1053/j.gastro.2008.09.016 279

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When transported into the mitochondria, the glycolytic end-product pyruvate is decarboxylated to produce acetyl-CoA by PDH complex, which reduces NAD + to NADH simultaneously