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The CUPRAC assay was carried out following the procedure described by Apak et al

The increase in iron undoubtedly exacerbates the occurrence of ferroptosis, and so, iron may act as a catalyst for the occurrence of ferroptosis, or serve a role as an inducer (112)

The precise mechanism(s) by which low GSH induces CYP2E1 and ALDH1A1 remain to be elucidated

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doi: 10.2337/db10-0193 74 DunsheeDRBainbridgeTWKljavinNMZavala-SolorioJSchroederACChanRet al

All cell lines except hTERT RPE-1 cells were cultured in DMEM (4.5 g l 1 glucose, Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U ml 1 penicillin (Thermo Fisher Scientific) and 100 g ml 1 streptomycin (Thermo Fisher Scientific)