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In Complex III, ubiquinone in its fully reduced form donates an electron to Cytochrome c 1 leaving behind an unstable ubisemiquinone semi-radical which favors leakage of electrons to O 2 , thereby generating O 2 (Murphy, 2008)

Biomedicines 11 (2), 481

The compounds of interest produced stable colorimetric signals that were observed by measuring the absorbance at a specific wavelength (Glucose Colorimetric Assay Kit II and Glutamine Detection Assay Kit: 450 nm) with a microplate reader (Infinite 200 PRO, Tecan)

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100 L of supernatant was mixed with 100 L methanol, centrifuged (42,500 g , 15 min), and filtered through a 0.22-m membrane filter prior to GC analysis

In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming