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Protect the plate from light

Tan YQ, Chen HW, Li J, Astragaloside IV

J Vasc Surg 13 , 128136 (1991)

Acknowledgements We thank Dr John Ravits, Dr Franca Cambi, Dr Haining Zhu and Dr Edward Kasarskis for providing us patient fibroblasts

Bentzley JP, Tomko RL, Gray KM

Glutathione-ethyl-ester (GSHee, Cayman, cat#14953) and N-acetylcysteine (NAC, Thermo Scientific, cat#160280250) were prepared as a 10 mM stock solution in PBS, filtered through a 0.22 m PVDF membrane (Millipore Sigma, cat#SH3007103), and added to fresh media at the desired final concentrations