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Drugs 45 (03), 392397

Zhongguo Zhong Xi Yi Jie He Za Zhi (2016) 36(5):55963

HIF-1 hydroxilated is associated to E3 ubiquitin ligase Von Hippel Lindau protein (VHLp) for its degradation, whereas in hypoxia condition stabilization and nuclear translocation occur, leading to oncogenes activation (192)

The membrane was blocked with 2% BSA overnight and was probed with antibodies against goat anti-FLAG (1:1000, Cell Signalling Technologies, D6W5B, #14793), alpha Tubulin (1:10,000, mAb, mouse) or rabbit anti-HA (1:1000, Cell Signalling Technologies) followed by a compatible HRP-linked secondary antibody