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Hyperglycemia acts as both an initiator of oxidative stress and a co-promoter of pathogenic molecular pathways, and cross-talk among these pathways induces ROS amplification via positive feedback, exacerbating oxidative damage, BRB impairment, iron dyshomeostasis and DR progression (157, 158)

X-Ray Powder Diffraction (XRPD) The crystallinity of the powders was evaluated using a benchtop X-ray diffraction instrument (Rigaku Miniflex 600 II, Woodlands, TX, USA) equipped with primary monochromated radiation (Cu K radiation source, = 1.54056 )

Selenium: This mineral is a structural component of glutathione peroxidase, an enzyme that helps glutathione do its job

Tissues were embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) by the University of Texas Southwestern Histo Pathology Core