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Furthermore, we compared the prediction accuracy of models developed using the 39 selected OTUs and all OTUs and found that the model developed using the selected features performed better in both the study and validation cohorts (Fig

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The collected samples were centrifuged at 3500 rpm for 15 min to separate the plasma and the separated plasma was transferred into micro centrifuge tubes (Sigma Aldrich, USA) to obtain cellular debris free plasma by further centrifugation at 12,000 rpm

USA 113 , E71E80 (2016)