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GHK-Cu stimulates the fibroblasts in the skin, which are responsible for producing collagen

This method offers several advantages over other routes of administration, such as oral or intramuscular, including faster onset, higher bioavailability, and the ability to administer substances that may be poorly absorbed or tolerated by other routes

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It was developed in Italy in the 1990s as an injectable wound-healing agent, and remains marketed there as Placentex

Cells were generally passaged twice a week