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1845 years of age

By studying the cellular and molecular basis of immune aging in nonmammalian vertebrates, such as teleosts, we aim to uncover conserved mechanisms of immune aging and identify new targets for interventions to enhance immune health during aging

Primary antibodies against -actin (1:1000 dilution, Santa Cruz Biotechnology, sc-47778), DDRGK1 (1:1000 dilution, Proteintech, 21445-1-AP), UFM1 (1:1000 dilution, Abcam, ab109305), UFL1 (1:1000 dilution, Proteintech, 26087-1-AP), EGFR (1:1000 dilution, Proteintech, 66455-1-Ig), and phospho-EGFR Tyr1068 (1:1000 dilution, CST, 2234S) were diluted in Primary Antibody Dilution Buffer (Beyotime Biotechnology, P0023A-500mL) and incubated with the membrane overnight at 4 C

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Again, correct insertion of the KEIO fragments in the marker free double mutants was verified using colony PCR with appropriate primers (Table 3)

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