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While refrigeration can't stop this, the overall slowdown of chemical reactions helps mitigate the damage

Finally, after nuclear staining with DAPI for 5 min, the NPCs were washed three times with PBS and imaged with a fluorescence microscope

AT8 and S2 formed more glycolytic xenografts, as demonstrated by higher [U- 13 C]lactate labelling, whereas AT5 xenografts were more oxidative with higher abundance of [ 13 C 2 ]glutamate (Fig