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9 Toxicology and side effects Current evidence reveals an intrinsic paradox between therapeutic efficacy and safety profiles of bioactive compounds - no identified pharmacologically active agent completely avoids non-specific off-target effects on healthy tissues ( in vitro study demonstrated that shikonin has potent cytotoxicity against 15 types of cancer cells through mitochondrial dysfunction and can induce apoptosis

In this regard, it has been reviewed recently that systemic administration of the DHP isradipine suppresses cytosolic calcium transients, increases mitochondrial mass and lowers mitophagy in dopaminergic neurons 54

doi:10.1097/00000658-198808000-00002

Dogs with IMHA are predisposed to thrombosis, particularly in pulmonary arteries (Carr et al 2002, DeLaforcade et al 2019), and hypoxic injury to tissues may contribute to hyperlactatemia and organ injury evident in many patients (MacNeill et al 2019)

Three independent experiments were performed and each individual samples were performed in triplicate

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