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The mRNAs were injected into each embryo at a concentration of 200 pg for Akt1, 150 pg for Akt2, and 100 pg each for Akt3a and Akt3b

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NP-MSCs tend to exhibit higher chondrogenic differentiation than BMSCs and ADSCs, considering that residual cell phenotypes are more readily available from MSCs isolated from specific tissues (Ukeba et al., 2020)

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Most protocols balance injection volume against precision, using 1-2ml for most reconstitutions

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